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Adherent cultured cells in each intervention group were stained with a BODIPY 581/591 C11 probe for 30 min at 37 C

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J Invest Dermatol (2006) 126:16008

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Central to all protocols in the preparation of PCLS is that the freshly retrieved whole lungs or individual lobes are infused with warm, low-melting agarose dissolved in buffer or tissue culture medium, at a concentration ranging from 0.5% to 3% (38) ( Figure 1 )