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Mitochondrial ROS-derived PTEN oxidation activates PI3K pathway for mTOR-induced myogenic autophagy

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Instead of utilizing 1-naphthylamine (5%), naphthyl ethylene diamine dihydrochloride (0.1% w/v) was applied in this investigation to modify the Griess-Illosvoy reaction

Immunofluorescent assay (IFA) of Ki67 and LGR5 further indicated a loss of LGR5 + ISCs in the crypt compartments of non-treated Veh controls and restored by IPA treatment at D7 when exposed to DSS, whereas no significant differences in immunostaining were seen between IPA- and Veh-treated mice at D0 (Fig