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These metabolites were introduced to our in vitro infection model, to analyze their cytotoxic potential in isolation

The correlation between PD and altered vascular function has prompted research into factors potentially involved in BBB dysfunction (Al-Bachari et al

For example, glucagon stimulates gluconeogenesis, which can upregulate ALT activity in the liver

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Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3) cultured in LB medium, after induction with 0.2 mM IPTG overnight at 16 C, the bacteria were pelleted by centrifugation at 4000 g for 10 min