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Briefly, in the NADH-coupled assay, 14 g (1.2 M) of detergent-purified protein was added to 150 L of reaction buffer containing 50 mM HEPES-KOH pH 8.0, 60 g/mL pyruvate kinase (Roche), 16 mg/mL lactate dehydrogenase (Roche), 4 mM phosphoenolpyruvate (Roche), 0.3 mM NADH, 3 mM ATP, 10 mM MgCl, 0.01% (w/v) LMNG, and 0.001% (w/v) CHS

For consistency in research, it's the most reliable method

Its ability to block estrogen where its harmful (breast, hypothalamus) and activate it where its beneficial (bone, liver) makes it one of the most intelligent and clinically validated hormone therapies available today

Osland, S

Magnsdttir S, Ravcheev D, de Crcy-Lagard V, Thiele I