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Progenitor cells were washed once in PBS before being resuspended in P3 Nucleofector Solution (5 10 5 cells in 20 l) and the cells added to the guideCas9 mixture

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Persistent fatigue, cognitive dysfunction, dizziness, pain, sleep disturbance, or autonomic symptoms may reflect several different mechanisms, including delayed diagnosis, neurologic Lyme disease, untreated co-infections, autonomic dysfunction, persistent infection in selected patients, or unrelated medical conditions

PMID 29765966 Inui S, Fukuzato Y, Nakajima T, Yoshikawa K, Itami S

The release of small concentrations of lactate over time had a very different effect as compared to the complete abolishment of activity observed when high concentrations of lactate were exogenously administered at once